Solid phase sandwich ELISA for the determination Human α-Synuclein in serum, EDTA plasma, CSF and cell culture supernatant. For research use only, not for use in diagnostic procedures.<br><br>
α-Synuclein is a 14.5kDa, soluble form protein which consists of 140 amino-acid residue and it is known as an intrinsically disordered protein. It is mainly expressed in cerebral neocortex, hippocampus, nigra, thalamus and metepencephalon. Majority of it exist in presynaptic terminal and nucleus of neuron cells and it is considered to involve with signal regulatory system of synaptic vesicle. α-Synuclein is main structural component of Lewy body which is an inclusion body exists inside of neuron cells in neurodegenerative diseases called “Synucleinopathy” such as Parkinson's disease, dementia with Lewy body and multiple system atrophy. It is considered that excessive accumulation of α-Synuclein is main cause of synucleinopathy due to inducing of shedding of dopamine-producing cells. The concentration of α-Synuclein in human blood or human CSF can be quantitatively measured by this ELISA kit.
- Assay Description:
- Overnight incubation (2 - 8°C) + 1 hour (2 - 8°C) + 30 min. (RT) = Overnight + 1 hour, 30 min. total incubation time
- Catalog number:
- 27740
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA using 2 kinds of highly specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Other names:
- Alpha-Synuclein
- Protocol:
- Sample types:
- Serum, EDTA plasma, CSF, and cell culture supernatant
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 0.16 - 10.00 ng/mL
- storage:
- 2 - 8°C
- sensitivity:
- 0.03 ng/mL
- Species:
- Human
- References:
