Enzyme immunoassay (ELISA) for the determination of Testosterone in rat and mouse serum or plasma. For research use only. Not for use in diagnostic procedures.<br><br>
Testosterone is a steroid hormone from the androgen group synthesized by the Leydig cells in the testes in males, the ovaries in females, and adrenal glands in both sexes. It exerts a wide-ranging influence over sexual behavior, muscle mass and strength, energy, cardiovascular health and bone integrity. Testosterone biosynthesis coincides with the spermatogenesis and fetal Leydig cell differentiation in the male rat. Several in vivo models including hormone-suppression, hormone-restoration and hypophysectomy were established for the study of the hormonal regulation of spermatogenesis by testosterone.<br><br>
In the Brown Norway rat, serum testosterone levels decrease with aging, accompanied by increases in serum FSH. The capacity of Leydig cells to produce testosterone is higher in young than in old rats. Testosterone secreted during late gestational and neonatal periods causes significant brain sexual dimorphism in the rat. This results in both sex-specific behavior and endocrinology in adults.
Analyses concerning the regulation of synthesis reveal that testosterone is able to regulate its own synthesis and indicate that this autoregulation is the result of rapid, specific inhibition by testosterone of 17 alpha-hydroxylase activity.
- Assay Description:
- 1 hour incubation (RT) + 30 min. (RT) = 1 hour, 30 min. total incubation time
- Catalog Number:
- IB79174
- Configuration:
- 96 Determinations, 12x8 removable strips
- Controls:
- Available upon request. Product Part # DEV99RC
- Design:
- Solid phase enzyme-linked immunoassay (ELISA)
- FDA Status:
- For research use only. Not for use in diagnostic procedures.
- MSDS:
- Please inquire
- Notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample Types:
- Serum and plasma
- Sample Volume:
- 10 µL
- Standards:
- 6 standards, ready to use
- Standard Range:
- 0 / 0.1 - 25 ng/ml
- Storage:
- 2 - 8 °C
- Sensitivity:
- 0.066 ng/ml
- Species:
- Mouse, Rat