Solid phase sandwich ELISA for the determination of Human Thioredoxin in serum, EDTA plasma, urine and cell culture supernatant. For research use only, not for use in diagnostic procedures.<br><br>
Human thioredoxin is a 120 kDa protein which consists of 105 amino acids and regulates the redox status in living bodies by reactions of two vicinal cysteines in its active site. It is known that thioredoxins are induced by stress such as ultraviolet, radiation, oxidant and viral infection, and act as a biological defense factor against stress, showing antioxidant effects and regulating activities of intracellular signaling molecules and transcription factors. Therefore, measuring thioredoxin is expected to monitor body’s stress status, and the study to improve the stress-resistance has been proceeding.
- Assay Description:
- 1 hour incubation (37°C) + 30 min. (37°C) + 30 min. (RT) = 2 hours total incubation time
- Catalog number:
- 27417
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA using 2 kinds of high specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Serum, EDTA plasma, urine and cell culture supernatant
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 3.91 - 250 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.43 ng/mL
- Species:
- Human
- References:
