Sandwich enzyme-linked immune-sorbent assay (ELISA) for the determination of TIMP-1 concentrations in serum, plasma, body fluids, tissue homogenate or cell culture supernates from rat. For research use only, not for use in diagnostic procedures.
- Assay Description:
- 90 min. incubation (37°C) + 60 min. (37°C) + 30 min. (37°C) + 30 min. (37°C) = 3 hours, 30 min. total incubation time.
- Catalog number:
- BE69210
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided.
- design:
- Sandwich enzyme-linked immunesorbent assay (ELISA) technique.
- FDA Status:
- For research use only, not for use in diagnostic procedures.
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Other names:
- Tissue inhibitor of metalloproteinase 1 (TIMP1), also known as Erythroid Potentiating Activity (EPA).
- Protocol:
- Sample types:
- Rat serum, plasma, body fluids, tissue homogenate or cell culture supernates.
- Sample volume:
- 100 μL of properly diluted unknown / determination.
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard.
- Standard range:
- 0 / 31.2 - 2000.0 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- <3 pg/mL
- Species:
- Rat
