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Tocilizumab (Actemra®) (mAb-based) ELISA Assay Test Kits

$1,730.00
SKU:
TM09021
Bulk Pricing:
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ImmunoGuide® Enzyme immunoassay (ELISA) test kit for the determination of free Tocilizumab (Actemra®) in serum and plasma.<br><br>

The drug Tocilizumab (trade name Actemra®) is a humanized monoclonal antibody (mAb) that specifically targets both soluble interleukin-6 receptor (sIL-6R) and membrane bound interleukin-6 receptor (mIL-6R) with high affinity, thereby preventing pro-inflammatory effects of IL-6. The specificity of this test system is achieved by using a monoclonal antibody (clon IG-7C84) for the coating of the microtiter plate and also as a conjugate.<br><br>

This ImmunoGuide Tocilizumab ELISA (mAb-based) is developed for the specific measurement of Tocilizumab in sera, plasma and other biological fluids by the advantage of using a site-directed IG-7C84 mouse monoclonal antibody (mAb) specific for Tocilizumab only. Binding of Tocilizumab to the solid phase, pre-coated with IG-7C84, is inhibited by recombinant human interleukin-6 receptor alpha (rh-IL6R) in a concentration dependent manner. Therefore, this Tocilizumab ELISA (mAb-based) measures the free form of Tocilizumab. The choice of specifically measuring the free form allows investigators to analyze the concentration-effect relationship. Due to the use of a mAb as catcher and also as detection reagent this test kit has an unmatched specificity. For research use only, not for use in diagnostic procedures.

Assay Description:
1 hour incubation (RT) + 1 hour (RT) + 10 min. (RT) = 2 hours, 10 min. total incubation time
Catalog number:
TM09021
configuration:
96 Determinations, 12x8 removable strips
controls:
None provided
design:
Enzyme immunoassay (ELISA) technique
FDA Status:
For research use only, not for use in diagnostic procedures
MSDS:
Available upon request
notes:
The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
Other names:
Actemra®
Protocol:

Protocol

Sample types:
Serum and plasma
Sample volume:
10 µL
standards:
5 standards, concentrated
Standard range:
0 / 6.0 - 200.0 ng/mL
storage:
2 - 8 °C
sensitivity:
2 ng/mL
Species:
Human
Products related:
Anti-Tocilizumab (Actemra®) ELISA
Additional info:

Pamphlet