Solid phase sandwich ELISA for the determination human TPO in cell culture supernatant. For research use only, not for use in diagnostic procedures. <br><br>
Thrombopoietin (TPO) is a 95 kDa glycoprotein consisting of 332 amino acids that was cloned and identified in 1994. It is produced mainly in the liver and secondarily in the kidneys and bone marrow. It is a hematopoietic factor which stimulates primitive blood cells to grow (proliferate) and evolve into platelet-producing megakaryocytes. TPO is also known as a ligand of c-MPL (Myeloproliferative leukemia protein) and is a key regulator of megakaryocytopoesis and thrombopoiesis. This ELISA kit can measure human TPO.
- Assay Description:
- Overnight incubation (4°C) + 1 hour (4°C) + 30 min. (RT) = Overnight + 1 hour, 30 min. total incubation time
- Catalog number:
- 27175
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA using 2 kinds of highly specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Other names:
- Thrombopoietin
- Protocol:
- Sample types:
- Cell culture supernatant
- Sample volume:
- 100 µL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 37.5 - 2400 pg/mL
- storage:
- 2 - 8°C
- sensitivity:
- 17.3 pg/mL
- Species:
- Human
- References:
