Sandwich enzyme-linked immune-sorbent assay (ELISA) for the determination of TGFβ1 concentrations in serum, body fluids, tissue homogenate or cell culture supernates from rat. For research use only, not for use in diagnostic procedures.<br><br>
Transforming growth factor beta 1 or TGFβ1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. In humans, TGFβ1 is encoded by the TGFβ1 gene. This gene contains 7 exons and very large introns, maps to 19q13.1-q13.3. TGFβ1 acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. The TGFβ1 is directly involved in the pathogenesis of bone marrow reticulin fibrosis in hairy cell leukemia. The expression of TGFβ1 in the early stages of DMD may be critical in initiating muscle fibrosis and suggested that antifibrosis treatment might slow progression of the disease, increasing the utility of gene therapy.
- Assay Description:
- 90 min. incubation (37°C) + 60 min. (37°C) + 30 min. (37°C) + 30 min. (37°C) = 3 hours, 30 min. total incubation time
- Catalog number:
- BE69208
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Sandwich enzyme-linked immune-sorbent assay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Other names:
- Transforming growth factor beta 1 or TGFβ1
- Protocol:
- Sample types:
- Rat serum, body fluids, tissue homogenate or cell culture supernates
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 15.6 - 1000.0 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- <1 pg/mL
- Species:
- Rat
