Mammalian target of rapamycin (mTOR), a member of the phosphatidylinositol 3-kinase-related kinase (PIKK), is a major regulator of translation, cell growth, and autophagy. mTOR constitutes two distinct complexes, mTORC1 and mTORC2, that differ in their subunit composition.Tti1/KIAA0406 was identified as a novel mTOR-interacting protein. Tti1 constitutively interacts with mTOR in both mTORC1 and mTORC2. Knockdown of Tti1 suppresses phosphorylation of both mTORC1 substrates (S6K1 and 4E-BP1) and an mTORC2 substrate (Akt), and also induces autophagy. And it has been confirmed that Tti1 binds to Tel2 also in mammalian cells and Tti1 interacts with and stabilizes all six members of the PIKK family of proteins (mTOR, ATM, ATR, DNA-PKcs, SMG-1, and TRRAP). Furthermore, it has been reported that knockdown of either Tti1 or Tel2 causes disassembly of mTORC1 and mTORC2. These results indicate that Tti1 and Tel2 are important not only for mTOR stability but also for assembly of the mTOR complexes to maintain their activities. For research use only, not for use in diagnostic procedures.
- application:
- WB
- Catalog number:
- 28117
- Datasheet:
- formulation:
- Lyophilized product from 1% BSA in PBS containing 0.05% NaN3
- immunogen:
- Synthetic peptide of the C-terminal part of Human Tti1/KIAA0408 (ASGQQNPYTTNVLQLLKELQ)
- MSDS:
- notes:
- For research use only, not for use in diagnostic procedures.
The datasheet for this product (see above) is intended to serve as an example only. Please refer to the datasheet provided with the antibody for precise details. - Other names:
- Please see datasheet
- Protocol:
- purification:
- Purified with antigen peptide
- size:
- 100 µg
- specificity:
- Please see datasheet
- storage:
- Lyophilized product, 5 years at 2 - 8 °C; Solution, 2 years at -20 °C.
- Species:
- Human
- Host:
- Rabbit
