Solid phase sandwich ELISA for the determination of Rat TNF-α in cell culture supernatant. For research use only, not for use in diagnostic procedures.<br><br>
TNF-α (Tumor Necrosis Factor-alpha) is a 17.5 kDa, 157 amino acids protein that is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and other target cells. TNF-α has been suggested to play a pro-inflammatory role and has been detected in synovial fluids of individuals who suffer rheumatoid arthritis. It is the primary mediator of immune regulation. The biosynthesis of TNF-α is tightly controlled being produced in extremely small quantities in quiescent cells, but is a major secreted factor in activated cells.
- Assay Description:
- Overnight incubation (4°C) + 30 min. (4°C) + 30 min. (RT) = Overnight + 1 hour total incubation time
- Catalog number:
- 27194
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA using 2 kinds of high specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Cell culture supernatant
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 56.25 - 3600 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 10.55 pg/mL
- Species:
- Rat
- Products related:
- The control set is available for sale on request
- References:
