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Vascular Endothelial Growth Factor (VEGF)(Mouse)

$634.00
SKU:
27102
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Solid phase sandwich ELISA for the determination of Mouse VEGF in EDTA-plasma, serum and supernatant of cell culture media. For research use only, not for use in diagnostic procedures. <br><br>
Vascular Endothelial Cell Growth Factor (VEGF) is a homodimeric protein initially purified from media conditioned by normal bovine pituitary folliculo-stellate cells and secreted by a variety of vascularized tissues. It was subsequently found to be identical to a vascular permeability factor (VPF), which was previously identified in media conditioned by tumor cell lines based upon its ability to increase the permeability of capillary blood vessels. The reported activities of VEGF include stimulation of endothelial cell growth, angiogenesis and capillary permeability. Human VEGF is a 38.2kDa homodimeric protein consisting of two 165 amino acid polypeptide chains. VEGF is expressed in many human tumor cells, including human adenocarcinoma, human pancreatic carcinoma, human hepatocellular carcinoma, renal cell carcinoma, fibrosarcoma, HL60 promyelocytic leukemia, GS-9L glioma and U937 lymphoma cells. In normal tissues, VEGF expression has been observed in activated macrophages, keratinocytes, hepatocytes, smooth muscle cells Leydig cells, embryonic fibroblasts and bronchial and choroids plexus epithelium, renal glomerular visceral epithelium and mesangial cells. In contrast to VEGF, VEGF-C, which was discovered as a VEGF-associated molecule, has been identified as a molecule that stimulates lymph vessel formation through VEGFR-3, its specific lymphatic endothelium receptor. The existence of human VEGF-C molecules having different molecular weights as a result of proteolytic processing has been reported. The kit is a complete kit for the quantitative determination of mouse VEGF.
Assay Description:
1 hour incubation (37°C) + 30 min. (4°C) + 30 min. (RT) = 2 hours total incubation time
Catalog number:
27102
configuration:
96 Determinations, 12x8 removable strips
controls:
None provided
design:
Solid phase sandwich ELISA using 2 kinds of high specific antibodies
FDA Status:
For research use only, not for use in diagnostic procedures
MSDS:

MSDS

notes:
The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
Protocol:

Protocol

Sample types:
Serum, EDTA plasma, supernatant of cell culture media
Sample volume:
100 µL of properly diluted unknown / determination
standards:
8 standards, serially diluted from 1 prepared lyophilized standard
Standard range:
0 / 62.5 - 4,000 pg/mL
storage:
2 - 8 °C
sensitivity:
10.98 pg/mL
Species:
Mouse
References:

References