Solid phase enzyme immunoassay for the combined determination of antibodies against PR3 and MPO in human serum. Each well is coated with native human proteinase 3 (PR3) plus native myeloperoxidase (MPO), both highly purified from human peripheral blood polymorphnuclear cells. Anti-PR3 and anti-MPO antibodies recognize specificially conformational epitopes only accessible on the native target antigen. For research use only, not for use in diagnostic procedures.
- Assay Description:
- 30 min. incubation (RT) + 30 min. (RT) + 30 min. (RT) + 5 min. (RT) = 1 hour, 35 min. total incubation time
- Catalog number:
- AE29016
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 1 positive control and 1 negative control, ready to use
- design:
- Solid phase enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Other names:
- Antibodies against proteinase 3 (PR3) and myeloperoxidase (MPO)
- Protocol:
- Sample types:
- Human serum
- Sample volume:
- 100 µL of properly diluted (1:101) unknown / determination
- standards:
- 6 calibrators, ready to use. Alternatively, a cut-off calibrator, ready to use, may be used for qualitative determination.
- Standard range:
- 0 / 3 - 300 U/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 1.0 U/mL
- Species:
- Human
