Enzyme immunoassay (ELISA) for the determination of Vasoactive Intestinal Polypeptide (VIP) in human EDTA plasma. For research use only, not for use in diagnostic procedures.
Vasoactive intestinal peptide (VIP) is a linear polypeptide containing 28 amino acid residues. The molecular weight is 3381. VIP is structurally related to secretin and to other members of the secretin family. The carboxyterminal amino acid of VIP (Asn) is amidated. Immunocytochemical studies have shown that VIP is present in neurons throughout the gastrointestinal tract, the central and peripheral nervous systems, the salivary glands and the pancreas. VIP stimulates water and bicarbonate secretion by the pancreas.
VIP possesses the capacity to relax smooth muscle. Administration of VIP produces vasodilation and pulmonary bronchodilation and relaxes the lower oesophageal sphincter and smooth muscle of the fundus of the stomach.
VIP is believed to play crucial roles in the regulation of intestinal motility and intestinal epithelial ion and water transport.
- Assay Description:
- 30 min (RT) + 60 min (RT) + 30 min (RT) + 30 min (RT) = 2 hours, 30 min. total incubation time
- Catalog number:
- IB59139
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 2 controls, lyophlized
- design:
- Enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Other names:
- VIP
- Protocol:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- MSDS:
- Available Upon Request
- Sample types:
- Human EDTA plasma
- Sample volume:
- 75μL
- Standards:
- 7 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 25 - 800 pg/mL
- storage:
- 2 - 8°C
- Species:
- Human
