Vitamin B12 as a trace element belongs to the biologically important chelate formers. The basic unit consists of a corrin ring with cobalt as a central atom. Cobalt is sixfold coordinated by four nitrogen atoms, one cyanide and a dimethylbenzimidazol group. <br> The existing detection procedures are mainly microbiological methods (1), but also HPLC and thinlayer chromatography, all of which are associated with a high amount of time and instrumentation. With the present test kit it is possible, to determine vitamin B12 quantitatively in vitaminated food (2) in a significantly faster way (2.5 to 4 hours inclusive sample pretreatment) compared with a conventional microbiological assay (24 to 48 hours).
This test is designed for detection of substances within food products. This is not a medical device and is not intended to diagnose or prevent any diseases or other conditions.
- Assay Description:
- 1 hour, 20 minute total incubation time
- Catalog number:
- DEB12E01
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Enzyme linked immunoassay (ELISA)
- Protocol:
- Sample types:
- Food
- Sample volume:
- 100 µL prepared sample
- standards:
- 6 standards, ready to use
- Standard range:
- 0 / 0.4 - 40 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.3 ng/mL
- Notes:
- This product is intended for research purposes only, not for use in diagnostic procedures.
The protocol shown for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the kit.
