Radioimmunoassay (RIA) for the determination of 25-hydroxyvitamin D3 and D2 (25-OH-D3 and 25-OH-D2) in serum. For research use only, not for use in diagnostic procedures.<br><br>
Vitamin D is the generic term used to designate Vitamin D3 or cholecalciferol and Vitamin D2 or ergocalciferol. Humans naturally produce Vitamin D3 when the skin is exposed to ultraviolet sunrays.
In the liver mainly, Vitamin D3 is metabolized into 25-Hydroxyvitamin D3 (25 OH D3) which is the main form of Vitamin D circulating in the body.<br><br>
25 OH D3 is a precursor for other Vitamin D metabolites and has also a limited activity by itself. The most active derivative is 1,25-Hydroxyvitamin D3, produced in the kidney (or placenta) by 1 α-hydroxylation of 25OHD3. 25OHVitamin D stimulates the intestinal absorption of both calcium and phosphorus and also bone resorption and mineralization. 25OH Vitamin D might also be active in other tissues responsible for calcium transport (placenta, kidney, mammary gland...) and endocrine gland (parathyroid glands, beta cells...).<br><br>
Vitamin D3 and Vitamin D2 are also available by ingestion through food or dietary supplementation.<br><br>
- Assay Description:
- 2 hour incubation (RT) + 1 hour (RT) = 3 hours total incubation time
- Catalog number:
- KIR1971
- configuration:
- Reagents sufficient for 96 determinations
- controls:
- 2 controls, lyophilized
- design:
- Radioimmunoassay (RIA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Other names:
- 25-hydroxyvitamin D3 and D2 (25-OH-D3 and 25-OH-D2)
- Protocol:
- Sample types:
- Serum
- Sample volume:
- 25 μL / determination
- standards:
- 6 calibrators, lyophilized
- Standard range:
- 0 / 10 - 100 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 1.5 ng/mL
- Species:
- Human
- radioactivity:
- 4.3 µCi (160 kBq)
