Solid phase sandwich enzyme-linked immunosorbent assay (ELISA) for the determination of HCP (Host Cell Protein) originated from cell culture medium using Corynex® System*. <br><br>
CORYNEX® is a novel protein/peptide expression system using Corynebacterium glutamicum (Corynebacterium Expression System). In the CORYNEX® system, C. glutamicum synthesizes and secretes recombinantly expressed proteins/peptides directly into the extracellular fermentation broth. Properly folded recombinant proteins can be recovered from the broth, which eliminates the processes of cell lysis and refolding. In the CORYNEX® system, host protein secretion has been minimized, and recombinant proteins may be transported extracellularly via one of two secretory pathways. Because C. glutamicum is a gram-positive bacterium, it lacks endotoxins, which simplifies the purification process. The CORYNEX® system has the advantage of a scalable, high cell-density fermentation process that can be used to manufacture commercially valuable proteins such as biopharmaceuticals, drug targets, and enzymes.<br><br>
This product is a complete ELISA kit for the quantitative determination of the host cell proteins derived from CORYNEX® culture broth, using rabbit antibodies.
The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals.
- Assay Description:
- 1 hour (37°C) + 30 min. (37°C) + 30 min. (RT) = 2 hour total incubation time
- Catalog number:
- 27800
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich enzyme-linked immunosorbent assay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Cell culture supernatant
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 7 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 31.25 - 2000.0 pg/mL
- storage:
- 2 - 8°C
- sensitivity:
- 3.57 pg/mL