Assay Description
90 min. incubation (37°C) + 60 min. (37°C) + 30 min. (37°C) + 30 min. (37°C) = 3 hours, 30 min. total incubation time.
Catalog number
BE69212
configuration
96 Determinations, 12x8 removable strips
controls
None provided.
design
Sandwich enzyme-linked immunesorbent assay (ELISA) technique.
FDA Status
For research use only, not for use in diagnostic procedures.
notes
The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
Other names
Tumor necrosis factor, formerly known as tumor necrosis factor-alpha or TNF-α.
Sample types
Mouse serum, plasma, body fluids, tissue homogenate or cell culture supernatants.
Sample volume
100 μL of properly diluted unknown / determination.
standards
8 standards, serially diluted from 1 prepared lyophilized standard.
Standard range
0 / 15.6 - 1000.0 pg/mL (body fluids, tissue homogenate or cell culture supernates).
0 / 7.8 - 500.0 pg/mL (mouse serum, plasma).
storage
2 - 8 °C
sensitivity
<1 pg/mL
Species
Mouse
